Application

Anti-Mouse IgG (whole molecule)−FITC antibody produced in goat has been used in flow cytometry.

The antibody may be used for immunofluorescent staining of mouse spleen cells at a working dilution of 1:64. A working antibody dilution of 1:40 was used to stain the conidia of Botrytis cinerea for immunofluorescence. The antibody was also used for immunofluorescence of primary luteal cell cultures incubated with primary antibody against CD45 and rhizoids of Chara globularis incubated with anti-human spectrin antibody.

Biochem/physiol Actions

IgG (immunoglobulin G) antibody has its function similar to IgM antibody in complement system activation. IgG participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG antibody consists of four subclasses : IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa respectively. The chains are interlinked with a disulfide bond.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections Anti-Mouse IgG (whole molecule)-FITC antibody is specific for mouse IgG. Goat anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. Following conjugation, unbound FITC is removed by extensive dialysis.

Immunogen

Purified mouse IgG

Packaging

0.5 mL in glass insert

1, 2 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.